MOUSE TRAPPING
نویسندگان
چکیده
منابع مشابه
Targeted gene trapping in mouse ES cells
Roland Friedel et al. report a method allowing efficient generation of targeted gene knockouts by homologous recombination in mouse ES cells. Current gene targeting techniques use vectors with a strong promoter to drive expression of a selectable antibiotic resistance marker, but such methods usually yield low homologous recombination frequencies because nonhomologous insertions (false positive...
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Hairless nude mice are immunodeficient because they lack a thymus. The nude gene has now been identified; it encodes a winged-helix transcription factor that is expressed specifically in skin and thymus.
متن کاملEnhanced gene trapping in mouse embryonic stem cells
Gene trapping is used to introduce insertional mutations into genes of mouse embryonic stem cells (ESCs). It is performed with gene trap vectors that simultaneously mutate and report the expression of the endogenous gene at the site of insertion and provide a DNA tag for rapid identification of the disrupted gene. Gene traps have been employed worldwide to assemble libraries of mouse ESC lines ...
متن کاملTrapping the mouse genome to hunt human alterations.
G lioblastoma multiforme (GBM) are the most common and aggressive adult primary brain tumors. Genetic alterations and their consequences in these malignant astrocytomas have been studied extensively and include (i) overexpression of growth factors and their corresponding receptors (fibroblast growth factor, epidermal growth factor, and platelet-derived growth factors), (ii) abnormalities of tra...
متن کاملNon-trapping Estimates near Normally Hyperbolic Trapping
In this paper we prove semiclassical resolvent estimates for operators with normally hyperbolic trapping which are lossless relative to nontrapping estimates but take place in weaker function spaces. In particular, we obtain non-trapping estimates in standard L2 spaces for the resolvent sandwiched between operators which localize away from the trapped set Γ in a rather weak sense, namely whose ...
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ژورنال
عنوان ژورنال: Science
سال: 1893
ISSN: 0036-8075,1095-9203
DOI: 10.1126/science.ns-22.554.148